Characteristics of fat body and transcriptional regulation of arylphorin receptor gene and yolk protein gene 1 in the adult oriental fruit fly, Bactrocera dorsalis

• Main Authors: Yu-Han Zuo, 左雨涵
• Other Authors: Kuang-Hui Lu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/59437899675535499781

碩士 === 國立中興大學 === 昆蟲學系所 === 97 === Insect fat body is responsible for storing nutrients, providing energy and metabolizing materials. Dissection and histological observation revealed that there were two forms of fat body in the adult abdomen of Bactrocera dorsalis (Hendel), i.e., white nodule-like “larval fat body” and thin sheet-like “adult fat body”. The amount of larval fat body was decreasing while adult fat body was increasing following adult growth. We further attempted to screen stage specific-expressed genes from the abdominal fat body of 0-day-old and 9-day-old adults using cDNA subtraction. The subtracted results showed that arylphorin receptor (ArR) gene expressed most abundantly in the 0-day-old adult fat body, about 15%; yet yolk protein gene 1 (yp1) was the major gene expressed in 9-day-old adult fat body. RT-PCR analysis demonstrated that ArR expressed greatly in larval fat body; in contrast, yp1 did not express in larval fat body, but specifically expressed in adult fat body of adult females. The transcriptional expression of ArR was decreasing, but yp1 was increasing with normal adult growth. In adult fat body, ArR and yp1 expressed less in starved females than that of control females. The duration of transcriptional expression of ArR was extended but that of yp1 was similar to the control after 20-hydroxyecdysone (20E) treatment. The expression of ArR was little lower after juvenile hormone analog, pyriproxyfen, treatment than control, but yp1 expressed earlier in the adult females at 16 h after pyriproxyfen treatment. ArR expressed higher, but yp1 expressed lower after an anit-juvenile hormone agent, precocene II, treatment than control. ArR expressed higher after precocene II and pyriproxyfen treatment than control, but the expression of yp1 after was similar to control. 20E and precocene II induced ArR expression that was inhibited by pyriproxyfen. There was no obvious effect of 20E on the expression of yp1 which could be induced by pyriproxyfen, but precocene II inhibited the expression of yp1.