| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 97 === In the past 20 years, lung cancer is the most common cause of cancer deaths worldwide and in Taiwan. Lung cancer also is the leading cause of cancer induced death in women in Taiwan. Metastasis is the major cause resulting in mortality of cancer patients which has been studied for many years. Over the past several dozen years, many genes and proteins involved in invasion and metastasis of cancers have been identified and validated. The microarray technique is the most popular and powerful high through-put screening tool to profile to gene expression. It has been applied in the identification of differentially expressed genes and molecular classification of diseases. In the previous study, we analyzed the gene copy number and gene expression of a lung cancer cell line model with varying degrees of invasiveness (CL1-0< CL1-1< CL1-5) by an integrated microarray approach that combines gene expression microarray and array CGH. We are interested to identify and focus the novel genes whose copy number or expression could inference cancer metastasis/tumorigenesis. SPANXA1 (sperm protein associated with the nucleus, X-linked, family member A1) is one out of approximately 300 candidate genes. The expression level of SPANXA1 is up to 100-fold in CL1-0 compared with that of CL1-5 assayed by RT-PCR. To explore the functions of SPANXA1 in cancerous phenotypes CL1-5 cells were transfected with a SPANXA1 expressing vector and evaluated by cell proliferation, cell migration, Matrigel invasion and colony formation assays in vitro as well as mouse metastasis assays in vivo. The results indicated that the induction of SPANXA1 could reduce cell invasiveness. In the other hand, immunostaining showed that SPANXA1 was predominately located at the nucleoplasm. Although SPANXA1 was expressed in melanoma and glioblastoma the biological significance is still unknown. This study is the first report to characterize the functions of SPANXA1 in lung cancers, to our best knowledge. Our study herein may provide the clues to interpret the mechanism of metastasis and develop the strategies of gene therapy.
|
|---|
