| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 97 === Urinary system is an important human excrete through the kidneys through the ureter, bladder, urethra and was discharged from the body. Once in this pathway, the urine presents the crystalline solid of mineralization, stop up the channel of urinating, form urine way calculus. Therefore, urolithiasis is an extensive general term, kidneys, ureter, bladder, urethra, etc. produced calculus. According to the analysis of chemical composition, the composition of urinary stones are calcium oxalate, calcium phosphate, calcium carbonate, uric acid and cystine, the most common is calcium oxalate stones, particularly account for 80%. Urolithiasis prevalence in Taiwan is about 9.6% belong to the high probability region, occurred in female and male ratio is 4.3 to 14.5. Typical symptoms of urinary tract stones are hematuria, renal colic, abdominal pain, urination, etc. tend to cause varying degrees of pain and cost of many medical and social resources. Although the diagnosis can be surgical treatment, but the cause and prevention of many unknown, and the protein was proposed to participate in the crystallization of mimerali- zation is an important molecule. This study is the use of calcium oxalate-induced rat model of renal changes in proteomics, hoping to identify proteins associated with the stone, the future of clinical trials can be used as a basis for further study the metabolic pathway or molecular mechanism. The results showed that ethylene glycol-induced rat stones, the urine in the lower free calcium concentration, although no significant differe-
nce (p = 0.059), but after that due to a large amount of ethylene glycol-induced oxalic acid, a large number of calcium oxalate combined with the in vivo lead to calcium oxalate crystal deposition in the renal tubular tissue, free urinary calcium decreased, and the results of the analysis of blood serum phosphorus concentration in the experimental group 9.45 ± 2.26 mg / dl was significantly lower than control group, 12.54 ± 1.25 mg / dl (p = 0.015). May be due to calcium oxalate and in vivo combination of calcium oxalate crystal formation, the effects of in vivo phosphorus-ion balance lead to lower serum phosphorus due to renal function and the relationship has to a certain extent, but in calcium oxalate stone-induced lower serum phosphorus concentration mechanism is not clear, the future will be able to track the results of this study for the upstream and downstream of the pathway, may be able to discover the in vivo calcium oxalate stone and the relationship between ion metabolism. Analysis of proteomics found in calcium oxalate stone albumin group was higher than the control group, so that after ethylene glycol-induced increase in albumin may be associated with renal calculi caused by renal tubular, glomerular tissue damage, inflammation response to or in connection with ion metabolism. In this study, in the blood, urine and proteomics analysis, are all reference to the biological diagnostic marker, it remained at a stage of animal experiments, there are still many hard work, research would be capable of unlimited extension of expectations by the results of this study applies to human medicine, and even as a stone marker test to discover the metabolic pathways and molecular mechanism.
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