| Summary: | 碩士 === 銘傳大學 === 生物科技學系碩士班 === 97 === Generally, cancer is presented in different pathological types of complexity, and the genetic characters are also shown as heterogeneity. On the other hand, the different pathogenic factor might result from mutations in the genetic message. Recently, molecular hybridization technologies have been commonly performed on cancer genetic studies. In which, the genetic materials were labeled with fluorescent dye as probes, and then hybridize onto the chromosome spread or interphase cells, after detection through the microscope, the scores will be correlated with the patients’ clinical outcome and further explore the relative significance of clinical, or attempt to searching the possibility of unknown disease associated genes. Herein, we used several different fluorescent probes, either derived from commercial available probes, for example, spectral karyotyping (SKY); or home made probes, which aim to map on certain genes, such as Fluorescence in situ hybridisation (FISH); or compared two different genetic materials for the purpose of screening whole genome alterations of the test samples, for example, Comparative genomic hybridization (CGH). Another goal of this study, since the occurrence of some cancers are rather low, therefore, to retrospectively studying the archive of such tumor bank, which commonly stored as formalin-fixed paraffin-embedded tissue (FFPE), become more important on those rare tumors. We compared the results from the different types of tumor samples, include fresh frozen tissues (UUT-TCC), cell lines (MALToma cell line) and FFPE tissue samples (GISTs) for a series of tests to challenge the limitation of traditional hybridization techniques. And we do succeed to get the results from nearly half of FFPE samples (GIST series).
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