Establish of the urinary PAH metabolite analysis method for assessing general population PAH exposure

• Main Authors: Yun-Chen Wu, 吳芸臻
• Other Authors: Chiung-Yu Peng
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/99539597239287788034

碩士 === 高雄醫學大學 === 職業安全衛生研究所 === 97 === Polycyclic aromatic hydrocarbons (PAHs) are a group of chemicals with two or more than two aromatic rings and widely distributed in air, in food, in soil, and in many occupational environments. PAHs form during incomplete combustion, such as smoking, cooking oil fumes. Some PAHs are considered as carcinogens or suspected carcinogens, such as benzo[a]pyrene and chrysene. Due to their ubiquitous presence and health effects, PAHs draw the public’s attention. Many studies have investigated the external and internal PAH exposure in occupational environments. Few studies examine the PAH exposure of the general population. Therefore, this study tries to establish a method to estimate internal PAH exposures of the general population. This study establishes the urinary PAH metabolite analysis method for assessing general population PAH. Four hydroxyl-PAH, which are 1-naphthol, 2-naphthol, 9-phenanthrol and 1-hydroxypyrene representing naphthalene, phenanthrene, and pyrene metabolites, respectively, are analyzed by High Performance Liquid Chromatograph/Fluorescence Detector. The linearity (expressing as R), limit of detection (LOD) and reproducibility (expressing as COV) are 0.996, 46 ~ 348 ng/L and 83.0 % ~ 107.9 %, respectively. Spot urine was collected from the test subject. Before analysis, 1.5-mL thawed urine was pretreated, purified, and condensed. The condensed extracts were quantitatively determined using the established method. The detection percentages of 1-naphthol, 2-naphthol, 9-phenanthrol, and 1-hydroxypyrene are 67.5 %, 5 %, 5 %, and 90 %, respectively. This method is good for detection of 1-naphthol and 1-hydroxypyrene. However, detection of 2-naphthol and 9-phenanthrol is fair, more advanced analysis techniques, such as high resolution gas chromatogram/mass spectrometry, are suggested to improve detection percentages of 2-naphthol and 9-phenanthrol.