Effects of grape seed extract on pharmacodynamics of pioglitazone in streptozotocin-induced diabetic rats

• Main Authors: Mei-Chuan Wu, 吳美娟
• Other Authors: Yaw-Bin Huang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/29113103612119102096

碩士 === 高雄醫學大學 === 藥學研究所碩士在職專班 === 97 === The incidence of diabetic population is increasing year by year in the worldwide mainly due to a change in the lifestyle and the type of diet. Recent survey data indicate that the certain proportion of diabetes mellitus had consumed dietary supplements (included a multivitamin/mineral and a herbal supplement). Approximately, half of these consumers also took prescription medicines at the same time, raising the potential of drug-dietary supplement interactions. The aim of this study was to investigate the effects of grape seed procyanidine in streptozotocin induced type 1diabetic rats and nicotinamide-streptozotocin induced type 2 diabetic rats. We used a single or simultaneous treatment of pioglitazone (20 mg/kg/day) and grape seed procyanidine (500 mg/kg/day) for 2 weeks in diabetic rats. Blood serum was analyzed for blood glucose, insulin, triglyceride and cholesterol levels. The results of this study showed that GSPE produced a significant reduction in plasma glucose in type 1 and type 2 diabetic rats. On the other hand, a simultaneous treatment of pioglitazone with GSPE were lowered the blood glucose concentrations compared with the treatment of pioglitazone alone. This result showed that simultaneous administration caused an additive effect. And the insulin level was increased following GSPE treatment in type 2 diabetic rats. However, pioglitazone with or without GSPE had no influence on the insulin levels of diabetic rats. In the lipid profile, GSPE was effective in lowering serum triglyceride levels in diabetic rats, and caused a significant reduction in type 1 diabetic rats. Pioglitazone was effective in lowering serum triglyceride levels in type 2 diabetic rats, and caused a significant reduction in type 2 diabetic rats. While coadministration of pioglitazone with GSPE had an significant additive triglyceride-lower effect in type 1 diabetic rats. Pioglitazone and GSPE either alone or coadministration did not affect total cholesterol in diabetic rats. The present results indicate that pioglitazone and GSPE possesses hypoglycaemic and hypolipicaemic potential. Therefore, the simultaneous administration had synergistic activity. Thus, from our study suggest that the simultaneous of GSPE as a dietary supplement with pioglitazone should be careful. Possibly, adjust the dose of prescription drugs.