| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所 === 97 === A field-amplified sample stacking and capillary electrophoresis with UV detection (CE-UV) is described for the determination of doxorubucin and doxorubicinol in human plasma. After sample pretreatment, field-amplified sample stacking method is applied for sensitive improvement. The sample was employed with an electrokinetic injection of 10 kV for 30 sec. CE separation of doxorubicin and doxorubicinol from human plasma was performed at 250C using a background electrolyte consisting of phosphate buffer (50 mM, pH 6.5) containing 16% ethylene glycol and methanol 60%. Using metformin as an internal standard (I.S.), the linear range of the method for the determination of doxorubicin and doxorubicinol was over 20.0-100.0 ng/mL. The LOD of the doxorubibin and doxorubicinol in human plasma was 5 ng/mL(S/N=3, 10 kV, 30 sec) and 10 ng/mL (S/N=3, 10 kV, 30 sec) respectively. For optimization of the procedure, the pH and strength of phosphate buffer, the volume of ethylene glycol and methanol would be discussed.
Application of the method to the determination of doxorubicin and doxorubicinol in human plasma proved to be feasible.
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