(I) Synthesis Histidine Peptide by SPPS and Modified PAMAM Dendrimers as Copper Ion Binders(II) Synthesis of Peptides-Modified Compounds as Specific Anti-Prostate Cancer Agent

• Main Authors: Hsin-Pei Wu, 吳欣蓓
• Other Authors: Chai-Lin Kao
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/24697642184140410957

碩士 === 高雄醫學大學 === 醫藥暨應用化學研究所 === 97 === There are many surface groups on the dendrimers surfaces. Histidine accesses the good binding affinity of metal ions, such as copper and nickel ions. In this thesis, we modified the surface groups of PAMAM dendrimer with different number of histidine peptide. The designed peptides were prepared by solid phase peptide synthesis (SPPS). Meanwhile, we modified the PAMAM dendrimer with histidine residue successfully, and the ability to bind copper was confirmed through the electrochemistry. In the future, these compounds may be a good metal binder. Meanwhile, prostate cancer is one of the top ten cancer incidence. The most important way to improve the drugs solubility and diminish side effect is to modify the drugs with specific peptides. For the prostate cancer, we choose the Bombesin (BBN) as our target peptides, the sequences are QWAVGHLM. At first, we synthesized the final compounds on solid phase, but the drug was decomposed in TFA which is necessary to collect peptide from resin. Thereafter, we have to synthesize and purify the peptide first then to modify the drug with the peptide. With this, we compared the solubility before and after the peptide modification by UV detection.