| Summary: | 碩士 === 高雄醫學大學 === 醫藥暨應用化學研究所碩士在職專班 === 97 === The synthesis of TTDA (3,6,10-tri(carboxymethyl)-3,6,10- triazadodecanedioic acid) derivative - TTDA-NP-NCS (2-methyl-4- isothiocynatephenol-3,6,10-tris(methoxycarbonyl)-3,6,10-triazadodecanedioic acid) and it’s Gd complex is the goal of this study. The NO2 group of TTDA-NP-4est can be hydrogenated to be a NH2 functional group. Furthermore, The NH2 group can transform isothiocynate functional group to conjugate special amino acid sequence or bio-active drug. The identification of TTDA-NP-NCS was conformed by NMR and LC-MS characterization. The ESI mass of Legumain peptide has a molecular ion peaks at 993.65 [M+H]+, which is similar to the Legumain peptide molecule weight. The ESI mass of TTDA-NP-NCS conjugated with Legumain peptide shows a molecular ion peaks at 1283.58 [M+H]+, which is similar to the actuality molecule weight. In the future, we will synthesize the Gd-TTDA-NP-Legumain complex and study on the Gd complex in vitro cell imaging, and investigate the Gd-TTDA-NP-Legumain as a MRI contrast agent ability.
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