| Summary: | 碩士 === 高雄醫學大學 === 天然藥物研究所 === 97 === Acacia confusa Merr. (Leguminosae), an evergreen trees and indigenous to Taiwan, is widely distributed on the Asia.
In present study, the heartwoods of Acacia confusa were collected and extracted with methanol. The extract was then partitioned to four fractions (n-hexane layer, n-BuOH layer, H2O layer and insoluble residue), among them, the n-BuOH layer showed anti HCV activity. Therefore, the subject was selected for further investigating in development of new anti hepatitis C virus agents.
The n-BuOH layer further separated by column chromatography (macroporous adsorbent resin, sephadex, silica gel) and high performance liquid chromatography, and gave twenty-six compounds, including thirteen flavonoids, Acacidin A (1), Acacidin B (2), 2R,3R,4R-4''-Methoxy-mollisacacidin (3), 2R,3R,4R-4''-Methoxy-3,3'',4,7,8-pentahydroxyflavan (4), Huazhongilexone (5), Isoliqiritigenin (6), Myricetin 3-O-??-glucopyranoside (7), Myricetin 3-O-??-rhamno-pyranoside (8), (+)-Dihydroquercetin (9), Quercetin (10), Luteolin (11), Kaempferol (12), 3,7,8,3''-Tetrahydroxy-4''-methoxyflavone (13); eight cerebrosides, 1-O-??-Glucopyranosyl-(2S,3R,4E,8Z)-2-N- [(2''R)-hydroxypalmitoyl]-4,8-octadecadiene-1,3-diol (14), 1-O-??- Glucopyranosyl-(2S,3R,4E,8E)-2-N-[(2''R)-hydroxypalmitoyl]-4,8-
octadecadiene-1,3-diol (15), Acaciconoside A (16), Acaciconoside B (17), Acaciconoside C (18), Acaciconoside D (19), Acaciconoside E (20), Acaciconoside F(21); one lignan， Lyonirsinol (22); two neolignans, Guajacylglycerin-??-coniferylether (23), Guajacylglycerin- ??-dihydroconiferylether (24); two glycerols, mixtures of 1-(24- Hydroxytetra-cosanoyl)glycerol (25) and 1-(26-Hydroxyhexa- cosanoyl)-glycerol (26).
Among them, the structures of compounds 1 and 2 are new skeleton, and compounds 3, 16, 17, 18, 19, 20, 21 are new. Structures of isolates were elucidated by spectroscopic data and chemical methods.
Compounds 16, 19, 20 showed better inhibitory effects on anti-HCV activity than the other compounds, among them, compound 19 has 52% inhibition at the concentration of 2.5 mM .
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