Development of Controller System for Sample Pre-treatment and PCR Microfluidic Chip

• Main Authors: Ying-liang Shen, 沈盈良
• Other Authors: Jr-Lung Lin
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/57684203522371129350

碩士 === 義守大學 === 機械與自動化工程學系碩士班 === 97 === Technologies and applications of microfluidic systems have been successfully developed since the concept of micro total analysis systems (µ-TAS) was introduced,. Various fluidic operations in microfluidic systems, such as sample preparation, sample injection, sample manipulation, reaction, separation, and detection, were successfully demonstrated in the biotechnological application. The advantage of microfluidic systems included of reduced reagent consumption and waste generation, fast reaction times, and a large surface-to-volume ratio, offering an intrinsic compatibility between the use of a microfluidic system and surface-based assay. This study aimed to design and fabricate a new microfluidic chip, which automatically controlled by a digital controlled system, integrated with pumps, a mixer, a magnetic separator and a PCR chamber. Here, a new micro-pump actuating 3-pair moving wall structures in a series has been demonstrated for sample transportation. The moving wall structures are activated pneumatically by six buried side chambers which deform the channel walls and generate a transportation of the sample streams to the detected region. Efficiency pumping are achieved using the developed controlled system to perform auto-transportation. Samples separated from fluid stream have been a challenging work for the application of the miniaturized biomedical systems. Generally, magnetic separator can be as simple as the application of the permanent magnet to the wall of the channel to cause aggregation. However, the permanent magnet is difficult to control of magnetic intensity as well as the function of switch on/off. Besides, 2-D microcoils were not only too difficult to be microfabricated but also that could be generated higher temperature to damage the biosample. Thus, a small-scale electromagnetic was introduced to generate a magnetic field to capture the magnetic beads to separate from the fluid streams. Finally, samples were amplified the RNA sequence for three PCR thermocycling temperatures by using ITO heaters. Experimental results showed that heated area of PCR chamber exhibited the temperature uniformity with a thermal variation within ±1?C. The developed chips will automatically detect the sample of virus. The success of the proposed microfluidic system will assure us to have a powerful tool to detect virus in an efficient and fast way.