| Summary: | 碩士 === 弘光科技大學 === 化妝品科技研究所 === 97 === Scopoletin (6-methoxy-7-hydroxycoumarin) is a pharmacologically active coumarin compound that has been isolated from several traditional Chinese herbs, such as Lycium chinense Mill and Angelicae dahuricae Radix. It also has been widely existed in the plant, such as Rutaceae Dictamnus、Rutaceae Murrayapaniculata. Several studies have shown that scopoletin show hepatoprotective activity, antihypertension, anti-bacteriae, down fever and relieve pain. In addition, It was shown that scopoletin show the anti-hemoglutination effect on the domestic rabbit. The aim of this research is to evaluate whether scopoletin whether has antioxidation and white efficacy.
Previous study reported that scopoletin has antioxidation activity. However, there is no report about interaction between scopoletin and other antioxidants such as vitamin C or vitamin E. Therefore, we aim to study possible synergistic antioxidation effects between scopoletin and vitamin C or vitamin E. Antioxidation activity was assayed by determining the DPPH radical scavenging activity , total phenol content, ABTS+ radical cation decoloriation and reducing power. The experimental results showed that DPPH radical capture ability is higher along with scopoletin concentration. The capture ability of scopoletin is also more obvious when combined with vitamin C or vitamin E. When scopoletin was combined with vitamin C, the total phenol content also increased in a dose-dependent pattern, whereas similar result was not found in the case of vitamin E. Additionally, scopoletin and vitamin C also showed remarkable synergistc effects on reducing power and the elimination of ABTS+.
Afterwards, the effects of scopoletin on mushroom tyrosinase activity, intracellular tyrosinase activity and melanin content of murine B16F10 melanoma cells were evaluated. Kojic acid was used as a positive standard in the above experiments.
The results showed that high concentration of scopoletin(2 mM~10 mM)could suppress mushrom tyrosinase activity in a dose-dependent pattern. However, when scopoletin combined with kojic acid, there is no obvious synergistic effect on the inhibition of tyrosinase activity. Interestingly, intracellular tyrosinase activity of B16F10 cells was suppressed by low concentration of scopoletin(25 μM, 50 μM, 100μM). Besides, the melanin content in B16F10 cells was also decreased by scopoletin in a dose-dependent pattern. Afterwards, we also evaluated the effects of scopoletin on protein content of melanin related proteins- Tyrosinae、Tyrosinase-related protein-1(TRP-1) and Tyrosinase-related protein-2(TRP-2) by western blot. The intracellular tyrosinase、TRP-2 was also decreased by scopoletin in a dose-dependent pattern. The further work will elucidate the whitening mechanism of scopoltin, and we will also evaluate the possibility of scopoletin applied in the antioxidation and whitening cosmetic formulations.
|
|---|
