| Summary: | 碩士 === 弘光科技大學 === 生物產業研究所 === 97 === The animal farming industry usually supplemented the antibiotics or drugs in feed to prevent the pathogens infection for animals. However, recently, the multi-drug resistance problem seriously affect in the animal therapy. Probiotics can enhance the immune system or produce the bacteriosin to reduce the infection by pathogens. Therefore, screening of the effective strains and use of them in multistrains or multispecis probiotics which strengthen the synergistic or symbiotic effect is important. These probictics will be beneficial to the animal farming industry. The purpose of this study was aimed to evaluate the efficiency of different combinations of multi-strains lactic acid bacteria (MiX-LAB) on the immune effect and the anti-salmonella infection on chickens and evaluate the efficiency of Strain L. acidophilus LAP5 inhibit different species salmonella infection on chicken.
Results showed that three Mix-LAB groups could significant enhance IgA levels in feces comparison with control group and in Mix-LAB group, Lactobacillus spp and bifidobacterium spp. in feces significant higher than control of chicken after different oral administration. Three Mix-LAB groups in comparison with control group, showed insignificant difference on the level of serum IgG and IgA in SPF chicken either after challenged but salmonella-specific IgM levels was significant higher than control. For Salmonella invasion experiment, Mix-LAB A and B probiotics were able to reduce Salmonella Typhimurium invasion into the liver of SPF chicken (p < 0.05) than the control group after the 7 day challenge.
L. acidophilus LAP5 was able to reduce S. pullorum invasion into the liver and spleen of SPF chicken (p < 0.05) than the control group after the 6 day challenge. LAP5 also could reduce S. Schwarzengrund invasion into the liver and spleen (p < 0.05) than the control group after the 3 or 6 day challenge. In S. Typhimurium challenge test, LAP5 was not able to reduce S.invasion to chicken. It may due to different Salmonella species have difference infection activeness.
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