The anti-inflammatory effect of dandelion extracts on macrophage cell line RAW 264.7

• Main Authors: Kai-Ting Zhan, 詹凱婷
• Other Authors: Chun-Hung Chiu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/82140053098018842764

碩士 === 弘光科技大學 === 生物科技研究所 === 97 === Dandelion have long been used as the folk medicine for their choleretic, diuretic and antirheumatic properties and are available today in market for a variety of health benefits. The anti-inflammatory activity of dandelion extracts has been confirmed in animal studies and dandelion flower fractions have been shown to possess both antioxidantive and antiangiogenic properties. Expanding upon from current understanding, we examined the effects of aqueous extract of dandelion（AED）and ethanolic extract of dandelion（EED）on lipopolysaccharide（LPS）- induced nitric oxide（NO）, tumor necrosis factor-�恁]TNF-�恁^and interleukin-6（IL-6）productions using macrophage RAW264.7 cells and revealed the possible mechanisms underlying. Both AED and EED displayed similar patterns on DPPH scavenging activity and reducing power, while only AED exhibited ferrous ion chelating ability. The contents of total phenolic compounds and flavonoids were higher in AED as compared with those of EED（262.2 mg and 110.0 mg vs. 152.5 mg and 49.5 mg）. The results indicate that AED and EED inhibited LPS-induced NO production in a dose-dependent manner. Furthermore, AED and EED also decreased the levels of IL-6 and TNF-��. In the presences of AED and EED inducible nitric oxide synthase（iNOS）protein and mRNA levels were declined significantly as compared with LPS induction alone. Both I�羠 and NF-�羠 expressions were also increased with the supplementation of AED and EED. These findings implicate that dandelion can be applied in the regulation of macrophage-mediated inflammatory diseases.