Protective Effect of Astaxanthin on Hydrogen Peroxide-Induced Cytoxicity in PC12 Cells.

• Main Authors: Jueen-Ya Chiou, 邱俊雅
• Other Authors: Chi-Huang Chang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/50783619919714948783

碩士 === 弘光科技大學 === 生物科技研究所 === 97 === Abstract The carotenoid pigment astaxanthin has important applications in the nutraceutical, cosmetics, food and feed industries. Astaxanthin is a strong coloring agent and a potent antioxidant .In this study we examined whether astaxanthin had a neuroprotection against H2O2-induced injury in PC12 cells. Calcium and lipid peroxidation play important roles in oxidative stress-induced cellular injury and apoptosis,which ultimately cause cell death. Pretreatment of PC12 cells with astaxanthin improved the cell viability, decreased malondialdehyde level and attenuated the intracellular Ca2+ concentration, and the reduced NO production in the H2O2-induced injury in PC12 cells.Furthermore, the [Caspase-3/pro-Caspase-3] and [Bax/Bcl-2] ratios were slightly low in H2O2-induced injury in PC12 cells after astaxanthin treatment . In summary, this study demonstrates that astaxanthin is able to relieve H2O2-induced oxidative stress and apoptosis in PC12 cells, at least in part, by Ca2+ antagonism and antioxidant mechanisms.