Screen of the Lactic Acid Bacteria Against Helicobacter Pylori Infection and Human Test of Commercial Yogurt Products for Improving Gastrointestinal Function

• Main Authors: Xing-Lu Lin, 林幸洳
• Other Authors: Chien-Ku Lin
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/95184307884765873903

碩士 === 弘光科技大學 === 生物產業研究所 === 97 === Lactic acid bacteria (LAB) are well documented and commercialized as probiotics for improving human health. The functional properties of probiotics are dependent on specific strain of LAB and need to be evaluated through animal experiments, even human tests. The purpose of present study is to screen the potential strains of LAB against Helicobacter pylori infection and evaluate the commercial yogurt products for improving gastrointestinal function in human. The study includes two sections. First, the lactic acid bacteria (LAB) against Helicobacter pylori were screed both in vitro and in vivo tests. LAB were isolated from different sources including infant stool, foods and Bioresourse Collection and Research Center (BCRC). To examine the probiotic properties of LAB, the capabilities of acid tolerance, bile tolerance, cell adherence and antagonistic activity were tested. The results indicated that A5Z2, BB5 and ABS1 were potential strains, and BB5 was selected for animal test. It was discovered that the number of H. pylori in mice stomach was significantly decreased after oral feeding BB5 strain. The immunomodulator cytokine TNF-αwas also significantly decreased. It is assumed that BB5 strain lowered the possibility of H. pylori infection and inflammation in stomach. This strain will be candidate for human test in the future. In order to determine whether ingestion of yogurt would alter the human intestinal bacterial flora and defecation function, ninety-nine healthy adult volunteers were recruited for a 5-weeks test. The average age of volunteers is 21.4±0.58 years old. This test included an initial two weeks without yogurt (control period), 2 weeks with two bottles (250ml each) of AB yogurt pre day (administration period), and one week without yogurt (follow-up period). The subjects were asked to record their own fecal characteristics and defecation frequency. The feces were collected for the analysis of bacteria flora, pH value and water content. The results indicated that ingestion of AB yogurt significantly increase the number of fecal lactobacillus, bifidobacteria and improve defecation frequency.