| Summary: | 碩士 === 輔仁大學 === 營養科學系 === 97 === The study was divided into two parts. In vitro, the enzymatic hydrolysis of the organs of sea cucumbers (OSC) were used to elucidate if there’s any antioxidative and angiotensin converting enzyme inhibition (ACEI) materials generated. In vivo, spontaneously hypertensive rats (SHR) were gavaged and feed with the hydrolysate or the different fraction of hydrolysate for a short-term to find out whether it have any antihypertensive effects .The hydrolysates obtained by proteolized with pepsin and chymotrypsin (PC) or Prozyme 6 and Flavourzyme (P6F). In vitro studies, results indicated that P6F hydrolysate had higher ACEI as well as higher activity in Trolox equivalent antioxidant capacity, 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity, and reducing power. The P6F hydrolysates were separated by ultrafiltration membranes, the fraction of MW<1 kDa was found having the highest ACEI and antioxidativity activity. The fraction of MW<1 kDa was further separated by gel filtration chromatography. The peptide with molecular weight of approximately 430 and 990 Da possessed the strongest activity of ACEI and antioxidation respectively. In animal studies, oral administration of P6F hydrolysate with the dose of 900 mg/kg bw to SHR exerted a significant lowering effect (p<0.05) on SBP after 3 h and lasted to 6 h. In addition, the fraction of MW<1 kDa of the hydrolysate had an antihypertensive effect significantly in SHR. In conclusion, the enzymatic hydrolysis of OSC may generate the hydrolysate with ACEI and antioxidative capacities, and exert an antihypertensive effect in SHR.
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