| Summary: | 碩士 === 輔仁大學 === 食品科學系 === 98 === The growth inhibition of H. pylori BCRC17021 by long-term coculture with each of three different probiotics and the effect of different dosages of Amoxicillin and Clarithromycin on the antibiotics tolerance of H. pylori BCRC17021 were studied. The inhibition of the growth of H. pylori BCRC17021 was studied by inoculating it with each culture of Bifidobacterium bifidum BCRC14630, Lactococcus lactis subsp. lactis BCRC12267, and Lactobacillus salivarius BCRC14759 in Brucella broth containing 10% bovine serum and incubating at 37℃ for 3-9 days microaerobically. The individual effect of three probiotics on urease activity of H. pylori BCRC17021 incubated for 18 h in Brucella broth containing 10% bovine serum and 0.1% urea showed that the urease activity in H. pylori BCRC17021 culture was significantly inactivated (p < 0.05). It was also found that the three probiotics cocultured with H. pylori BCRC17021 at pH 5.0, 6.0, and 7.0 for 9 days were able to inhibit the growth of H. pylori BCRC17021 significantly (p < 0.05). The three probiotics were able to inhibit the growth of H. pylori BCRC17021 after addition of 16.7 mg/L Amoxicillin and 8.3 mg/L of Clarithromycin to Brucella broth containing 10% bovine serum at pH 5.0, 6.0, and 7.0 (p < 0.05). The growth inhibition of H. pylori BCRC17021 with cell count of 104-106 CFU/mL was observed by 9-day coculture of H. pylori BCRC17021 with each culture of probiotics at the same level. Greater inhibition effect was obtained when higher concentration of proboitics was employed. The inhibitory effect of probiotics in pH 5 was better than that in pH 6 and 7. Significant inhibition effect (p < 0.05) on H. pylori BCRC17021 was achieved in the presence of Amoxicillin and without cultures washing during 9-day coculture incubation.
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