| Summary: | 碩士 === 輔仁大學 === 食品科學系 === 98 === Lycium barbarum L., Zizyphus jujuba Mill. and Gynostemma pentaphyllum Mak. are traditional Chinese herbal medicines, rich in many functional components responsible for various biological activities. These herbal medicines have been widely applied in the development of pharmaceutical products and functional foods. The objectives of this study were to investigate the effects of polysaccharide from L. barbarum, Z. jujuba and
G. pentaphyllum on antimutagenicity by Ames test and on cytotoxicity to hepatoma (HepG-2) and colon (Caco-2) cancer cell lines using MTT cell viability assay. The hot water extracts of crude polysaccharide, neutral and acidic polysaccharides from the three herbs have been tested for their antimutagenic properties against direct-acting mutagen of 4-NQO and sodium azide and indirect-acting mutagen of B[a]P and 2-AF to Salmonella Typhimurium strains TA98 and TA100. Four different dosages of 0.5, 1.0, 2.5 and 5.0 mg/plate were used, and the polysaccharides tested did not show any toxicity and mutagenicity. All the tested crude / purified polysaccharides from the three herbs showed antimutagenicity effect. Results also showed that crude / purified polysaccharides from L. barbarum and G. pentaphyllum exhibited better effect against 4-NQO to TA98. Maximum antimutagenic activities of the crude / nutral / acidic polysaccharides from
L. barbarum were 67%, 59.3% and 49.8% at 5 mg/plate, and that of crude / neutral / acidic polysaccharides from G. pentaphyllum were 50.7%,57.2% and 44%. Data of MTT assay indicated that crude polysaccharide at low concentrations of 2~6mg/mL did not have significent cytotoxicity to HepG-2 and Caco-2 cells (p>0.05), with 90~100% viability.
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