Using a Bioinformation Approach Search to Splice Sites Regulated by RNA Editing

• Main Authors: Yu-Chan Huang, 黃于展
• Other Authors: Fang Rong Hsu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/05156673827106335144

碩士 === 逢甲大學 === 資訊工程所 === 97 === Because the technology of the molecular biology and bioinformatics have been advance, the previous studies that analyzed the large-scale genome sequence had not been difficult. The previous studies indicated there are a variety of genetic diseases and cancer are related to specific protein. The structure and function of proteins depends on the amino acid sequences. Amino acid sequences are come from DNA sequences information. Therefore, this is an important study which we recognized DNA contained the genetic code for the content. There have two important processes in transcription, and these processes were alternative splicing and RNA editing. In recent years, the study indicated human genes experienced alternative splicing events about 40% to 60% Alternative splicing is a study which is a valuable part in the molecular biology. In recent years, some studies reported the alternative splicing events regulated by RNA editing. Due to adenosine-to-inosine (A-to-I) of the RNA editing, Inosine (I) is read in translation and will be encode as Guanosine (G). This phenomenon maybe affected the splicing type GT-AG, then arose alternative splicing. Therefore, we hope to use the bioinformatics approach to identify splicing sites regulated by RNA editing in human, mouse, rat and zebrafish. We discovered two RNA editing sites in human gene. There are six RNA editing sites in mouse gene, thirteen editing sites in rat gene, and zebrafish has sixteen editing sites. We also compared cross-species by a multiple alignment tool. We observed some A-to-I editing sites are a geneomically encoded G, and some sites are splice sites, other sites are in exon. Consequently, we consider evolution of species related by RNA editing.