| Summary: | 碩士 === 逢甲大學 === 資訊工程所 === 97 === Because the technology of the molecular biology and bioinformatics have been
advance, the previous studies that analyzed the large-scale genome sequence had not
been difficult. The previous studies indicated there are a variety of genetic diseases
and cancer are related to specific protein. The structure and function of proteins
depends on the amino acid sequences. Amino acid sequences are come from DNA
sequences information. Therefore, this is an important study which we recognized
DNA contained the genetic code for the content. There have two important processes
in transcription, and these processes were alternative splicing and RNA editing. In
recent years, the study indicated human genes experienced alternative splicing events
about 40% to 60% Alternative splicing is a study which is a valuable part in the
molecular biology. In recent years, some studies reported the alternative splicing
events regulated by RNA editing. Due to adenosine-to-inosine (A-to-I) of the RNA
editing, Inosine (I) is read in translation and will be encode as Guanosine (G). This
phenomenon maybe affected the splicing type GT-AG, then arose alternative splicing.
Therefore, we hope to use the bioinformatics approach to identify splicing sites
regulated by RNA editing in human, mouse, rat and zebrafish. We discovered two
RNA editing sites in human gene. There are six RNA editing sites in mouse gene,
thirteen editing sites in rat gene, and zebrafish has sixteen editing sites. We also
compared cross-species by a multiple alignment tool. We observed some A-to-I
editing sites are a geneomically encoded G, and some sites are splice sites, other sites
are in exon. Consequently, we consider evolution of species related by RNA editing.
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