Effect of surface modification on cell adhesion and detachment on Poly(methyl methacrylate) surfaces

• Main Authors: Li-Qun Lai, 賴力群
• Other Authors: Chih-Hsin Shih
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/82917683754436105152

碩士 === 逢甲大學 === 化學工程學所 === 97 === Abstract Surface modification on polymeric materials plays an important role on tissue engineering and biomaterials. The surface properties, such as porosity, crystallinity, presence and distribution of electrical charges, functional groups, hydrophilic and hydrophobic properties, are very critical in determining biocompatibility of the polymeric materials. In this work we study the effect of various surface modification methods on PMMA (polymethylmethacrylate). The surface modification methods include wet chemical (poly-L-lysine、collagen) and plasma (O2、CF4) methods. Biocompatibility of the modified surface to the MCF-7 cells was evaluated by cell viability test using MTS assay and the adhesion/detachment of the MCF-7 cells to the modified surface is also conducted under various flow conditions. It is found that modification of PMMA surface can greatly enhance cell proliferation and adhesion than tissue culture dishes. Especially with the proper treatment of CF4 Plasma, the MCF-7 cell proliferation and adhesion to the surface can be greatly improved. Keywords: surface modification, microchannel, cell viability, cell adhesion/detachment.