Self-Assembled on Various Substrates Using Liquid-Cell Atomic Force Microscopy

• Main Authors: Meng-hao Yao, 姚孟豪
• Other Authors: Wei-Li Yuan
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/35872821054841063959

碩士 === 逢甲大學 === 化學工程學所 === 97 === In this thesis, we studied the self-assembling behavior of gold nanoparticle or antibody monolayer prepared on mica, single crystalline silicon, ITO, and graphite surface. Firstly the size of gold nanoparticle was analyzed by particle sizer and secondly the morphology and average thickness of gold nanoparticle or antibody on different substrates were characterized by atomic force microscope (AFM) in liquid. Results show that the sizes obtained from the laser particle sizer were 2 nm and 2-5 nm for gold particles prepared by methods of salt reduction and phase transfer, respectively. Adsorbing and self-assembling on mica, silicon chip, and graphite substrates, it was found that the rabbit anti-mouse IgG-FITC monolayer gives a height of 5-10 nm. It was also found that only 5 min was needed to form self-assembled monolayers of 3-aminopropyl trimethoxysilane (APTMS), gold nanoparticle, and IgG-FITC on silicon chip, ITO, and mica surface. In addition, it was proved that it is evident for the surface hydrophobicity to be modified with monolayers by measuring the contact angles of APTMS, adiptic acid, and gold nanoparticle on substrate.