Development of Immunoseparation Method for The Rapid Capture of LAB in Beers

• Main Authors: CHE-HUNG YEH, 葉哲宏
• Other Authors: SHIH-CHIEH SLEE
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/74035838119266814968

碩士 === 大葉大學 === 分子生物科技學系碩士班 === 97 === ABSTRACT In recent years, developement of the molecular diagnostic methods for bacteria identification has been noteworthy. Conventional methods for detection of LAB are laborious and time consuming and may require 1 week . Of the molecular dctection methods, when many LAB species are subjected to identification, the biochip method could be used. To establish the online monitoring system for the detection of the LAB cells in beers during brewing, in my thesis work, the immunomagentic beads method specific for LAB species in beers will be developed for the collection and concentration of lactic acid bacteria cells including Lactobacillus brevis, L. casei, L. plantarum and Pediococcus damnosus. These four lactic acid bacteria may spoil the beer and should be detected online during the brewing processes. The antibody to these LAB species was obtained from immunized rabbit and then purified. The purified antibody was linked to magneticbead. After obtaining the immunomagnetic bead, the LAB cells in beer were captured and detected using the biochip from Dr. Chip Company. By this way, the online monitoring system for detection of LAB during the beer brewing process could be establish.