Study of Electrospun Poly (o-methoxyaniline) Nanofibers for Tissue Engineering

• Main Authors: Mu-Fong Hsieh, 謝沐峰
• Other Authors: Wen-Tyng Li
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/19622953230243358309

碩士 === 中原大學 === 醫學工程研究所 === 97 === Abstract Conducting polymers have both the physical and chemical characteristics of organic polymer as well as the conductivity of metals, which make them suitable to be used as photoelectric or biosensing materials. The purpose of the research was to study the biocompatibility of electrospinning poly o-methylaniline nanofiber and the feasibility for tissue engineering application. According to the SEM images, the diameter of poly o-methylaniline nanofiber was between 200 and 300 nm. Contact angle was 104 ± 8¬ o, which made its surface hydrophobic. Thermogravimetry analysis revealed that Tg was between 300 and 600 oC. The material had redox potential by cyclic voltammetry analysis. According to the ISO 10993-5 specification, we found that cytotoxicity of electrospinning poly o-methylaniline nanofiber was under the scope of acceptance prior and after electrical stimulation. From cell attachment and proliferation study, L929 fibroblasts had poor cell adhesion and proliferation on electrospinning poly o-methylaniline nanofiber compared with C2C12 myoblasts. By studying the gene expression of myoD, myogenin, myf-5, MRF4, myogenic differentiation of C2C12 cells was enhanced. In vitro biocompatibility studied by macrophages found that electrospinning poly o-methylaniline nanofiber induced the release of nitric oxide and inflammation cytokines. LDH release further demonstrated that the material caused some cell damage which elicited minor inflammation. Protein adsorption assay showed that human serum albumin and human plasma fibrinogen reached 80 μg/cm2 after 48 hr. In vivo biocompatibility was performed by implanting electrospinning poly o-methylaniline nanofiber in the back of Wistar rats subcutaneously. Some neutrophil and macrophages appeared after 1 and 4 weeks, and blood vessels were observed after 8 and 12 weeks. Our study suggested that electrospinning poly o-methylaniline nanofiber had relatively low toxicity, good biocompatibility, low protein adsorption and low immunogenicity. Although the material may not be suitable for fibroblast attachment, it is suitable for the attachment and growth of myoblasts. Electrospinning poly o-methylaniline nanofiber may be applied in muscle tissue engineering applications.