Effects of Nano-Isoflavones on MC3T3-E1 Cells Activation and Bone Formation of Postpartum Mice

• Main Authors: Jenn-Jou Chen, 陳鎮洲
• Other Authors: Walter-H Chang
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/87591489635337262606

碩士 === 中原大學 === 醫學工程研究所 === 97 === During pregnancy, maternal hormonal changes and the lost of calcium will make maternal bone mass lower. Successive pregnancies and deficient Ca nutritional status could transform these transient periods of women’s life in a deleterious event that could lead to osteopenia or even osteoporosis. In the study, we used postpartum mice as experimental model and examined the therapeutic efficacy of the traditional isoflavones, the nanoscaled isoflavones. To examine the pathological influence on osteoblasts for nano-isoflavones, the experimental design in this research mainly divide into five parts: (1) In vitro study, the activity of osteoblasts were evaluated by the methods of MTT assay. (2) Establish the osteoporosis model by postpartum mice. (3) Measure the change of BMD by DEXA. (4) Utilized the method of cross-linkers to conjugate the activative component (genistein and estradiol) on the surface of QDs. And finally (5) Utilized the fluorescence microscopy to observe the pathway on cells. In conclusion, Our results show that nano-isflavones could prevent bone resorption diseases by the promotion of osteoblasts activation. We also confirmed the specificity of genistein by targeting of QDs.