Human Nonmetastatic Clone 23 Type 1 and Human Telomerase Reverse Transcriptase in Cancer of Uterine Cervix

• Main Authors: Chien-Gang, 徐千剛
• Other Authors: 柯俊良
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/63061787871513455902

博士 === 中山醫學大學 === 醫學研究所 === 97 === Human nonmetastatic clone 23 (nm23-H1) and human telomerase reverse transcriptase (hTERT) may be separately involved in tumor progression of uterine cervix. Moreover, we found normal cervix and cervical low-grade intraepithelial neoplsia (low-grade CIN) samples had concurrent low expression of hTERT and nm23-H1, whereas high-grade CIN [CIN2 (moderate dysplasia) and CIN3 (severe dysplasia, carcinoma in situ)] SCC samples had concurrent high immunoreactivity. However, hTERT immunoreactivity seemed to have no significant association with clinicopathological variables of cervical cancer. On the contrary, only deep stromal invasion was significantly associated with high nm23-H1 immunoreactivity based on our limited cervical tissue samples. A high cumulative recurrence hazard was demonstrated for the high nm23-H1 expression group. Therefore, using real time polymerase chain reaction and tissue microarray of cervical samples, we further demonstrated that nm23-H1 mRNA expression and H score (median H scores: 2.0 vs. 0.3, P = 0.001) were higher in cervical cancer tissues than normal counterparts, respectively. Nm23-H1 expression was significantly associated with depth of stromal invasion (P = 0.003), tumor diameter (P = 0.044) and cell differentiation (P = 0.025). Furthermore, positive nm23-H1 expression was significantly associated with poor overall survival.