Establish the Therapeutic Effects of Neurotropic Factors in Traumatic Rat Brain Injury Model

• Main Authors: Dar-Yu, 楊大羽
• Other Authors: Ming-Chih Chou
• Format: Others
• Language: en_US
• Online Access: http://ndltd.ncl.edu.tw/handle/77744005299861082352

博士 === 中山醫學大學 === 醫學研究所 === 97 === From the Head and Spinal cord injury research held in the First International symposium of the Epidemiology of head and Spinal Cord Injury, an incidence of severe head injuries is 200-300/100,000 per year for the total world population of 5.4 billion(Jennett and frankowski,1990). This is an enormous global burden with regard to both loss of manpower and cost. We went to test the potential pathophysiological roles of traumatic ischemia in brain infusion. Acute brain ischemic is usually caused by thromboembolic occlusion of a cerebral artery [58]. Thrombolytic treatment for ischemic is directed to recanalize the occluded cerebral artery to reperfuse the ischemic brain areas. Intravenous or intra-arterial administration of tissue plasminogen activator (tPA) for treatment of acute ischemic stroke has recently been reported.. It suggests that cerebral microvascular permeability might be increased after high-dose tPA treatment. We found that intravenous infusion of 5 and 7.5 mg/kg tPA significantly reduced the cerebral infarction caused by ischemia/reperfusion but 10 mg/kg aggravated it. Increasing evidence suggests that oxidative stress can increase the occurrence of edema, cytokine expression, MMP activation, and apoptosis. It is well known that a loss of vascular integrity results from the degradation of the basal lamina and extracellular matrix.these findings demonstrate the detrimental effect of PUFA such as AA and DHA in post-ischemic progression and brain injury after cerebral I/R is associated with augmentation of cerebral I/R-induced alterations, including oxidative changes. Lastly,stem cell and gene therapy treated group. Rate will be subjected to a lateral fluid percussive brain injury（~5 atm）. TTC will be examined at the light microscopic levels. The results of this proposed experiment should be able to clarify the roles of ischemia in traumatic brain swelling and the effect of stem cell with genes therapy infusion in preventing the secondary vasogenic edema. The preliminary result showed the restoration of brain of neurostem cell and some degree of function recovery,We used immunochemical stain to evaluate the change of volume of trauma and effect of neurostem cell and gene therapy