The Growth-Inhibitory and Apoptotic Effects of Saponins of Keelung Yam (Dioscorea Pseudojaponica Yamamoto) on Cancer Cell Lines

• Main Authors: Chia-Hsi, 陳佳禧
• Other Authors: 王進崑
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/29067404401152107862

碩士 === 中山醫學大學 === 營養學研究所 === 97 === Saponins, steroid saponins, which are rich and play the most important physiologically active compounds in yam. The studies of steroid saponins of other plants found that glycone on saponins played an important physiological activity. Because of the structural differences between saponin glycosides with different forms and sapogenin resulting from the removal of glycone by hydrolysis on saponins , caused the different physiological effect. The research is little and unclear about the physiological metabolism and biological activity caused by structural differences between yam steroid saponins and sapogenin. In this study, therefore, the furostanol glycosides (FUG) and spirostanol glycosides (SPG) with different forms of glycoside, and diosgenin (DIO) with no glycone were isolated from yam (Dioscorea Pseudojaponica Yamamoto in Taiwan) that were investigated the effect and mechanism on anti-cancer for the structurally different Saponins and sapogenin. The study found that the rank for the anti-cancer effect on A549 lung adenocarcinoma cells、Hep G2 hepatocellular carcinoma cells and MCF-7 breast adenocarcinoma cells by yam steroid sapogenin and saponin is SPG ＞ DIO ＞ FUG, all are rather better than anti-cancer drugs for 5-fluorouracil and methotrexate on anti-cancer effect. The rank for the selective toxicity and safety on clone-9 normal rat liver cells and HS68 normal human foreskin fibroblasts is FUG > DIO > SPG. Yam steroid saponins and sapogenin are resulting in MCF-7 cells apoptosis by arrested the G0/G1 phase, increased the p53 protein with promoting apoptosis and decreased the AKT protein with anti-apoptosis and the BRCA-1 protein with repairing DNA. And, SPG has the excellent anti-cancer effect on MCF-7 cells may be due to induced much of the p53 protein in the early treated stage.