| Summary: | 碩士 === 嘉南藥理科技大學 === 化妝品科技研究所 === 97 === This research aims to investigate whether the vegetable extracts contain high activities of enzymatic antioxidants (superoxide dismutase, SOD) and assess the potentials of these extracts applied in cosmetic industry.
Sixteen common vegetable wastes were collected for activity assay, including Raphanus sativus leaf , Phaseolus lunatus shell, Brassica rapa chinensis leaf, Apium graveolens leaf, Brassica oleracea L. var. botrytis L. stalks, Brassica juncea leaf, Asparagus officinalis peel, Zizania latifolia shell, Chrysanthemum coronarium leaf, Brassica rapa leaf, Brassica oleraceae L. var. capitata L. leaf, Brassica chinensis leaf, Brassica campestris leaf, Brassica pekinensis leaf, Phyllostachys edulis shell, and Morus alba leaf. All vegetable wastes were water-extracted and filtered to harvest extracts, respectively.
Extracts were then examined by SOD activity staining analysis via a series of NBT verification proceduces, cell viability assay with a tetrazolium dye, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), gelatin-based zymography, and a cell-based assay, using 2'', 7''-dichlorofluorescin (DCFH) oxidation.
To begin with, SOD activity staining analysis revealed that three vegetable extracts [ i.e. the extract of Brassica oleracea L. var. botrytis L. stalks (BOBE), the extract of Brassica oleraceae L. var. capitata L. leaves (BOCE), and the extract of Morus alba leaves (MAE) ] were of significant activities and the rate for inhibiting free radicals of SOD exceeded 96% .
Secondly, the MTT assay confirmed that high concentrations of MAE declined the cell survival rate to 60-70%, but such declination did not apply to other vegetable extracts aforementioned.
Thirdly, BOBE and BOCE did not inhibit the activities of MMP-2 and MMP-9, whereas MAE showed significant MMP-9 inhibition during 48 hours treatment.
Moreover, the activities of non-enzymatic antioxidants in BOCE and MAE exhibited 70~80% of inhibition in DCFH oxidation at 6.62 mg/ml and 6.27 mg/ml, respectively.
|
|---|
