Effect of pretreatment with S-adenosylhomocysteine on heatstroke in rats

• Main Authors: Yi-Chun Yeh, 葉怡君
• Other Authors: Chia-Chyuan Liu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/katvhz

碩士 === 嘉南藥理科技大學 === 營養與保健科技研究所 === 97 === Many studies indicated that plasma high levels of S-adenosylhomocysteine (SAH) might be mediated in neuronal injury or death, and it could be also one of the high risk factor in cardiovascular disease. Workers in high temperature and humidity were easy to induce heatstroke formation. Studies found that the heatstroke patients and animals all displayed the central ischemia, hypoxia, neuronal damage, and high mortality. Many literatures suggested that the patients of cardiovascular diseases might be easier to evoke heatstroke induction. However, there is less attention to investigate what role of the SAH played during heatstroke induction, and whether plasma high levels of SAH could be also one of high risk factor in heatstroke induction. So far, there is no one strategy can be to tall therapy the heatstroke- induced damage. Consequently, it seems more important to prevent the heatstroke induction. The main purpose of this study is to observe the effects of pretreatment with SAH on heatstroke induction and heatstroke- induced damage by a rat model. Rats under anesthesia were exposed to an ambient temperature (42℃) to induced heatstroke. We observe effects of pretreatment with high concentration 2 ml SAH (5 mg/ml in 5?s DMSO) or 2 ml 5?s DMSO on heatstroke. Our results showed that rats with high concentration SAH treatment were significantly shrunk the duration time of heatstroke induction. The situations of heatstroke- induced cerebral ischemia, hypoxia and neuronal damage were apparently revealed ahead of time in rats with SAH pretreatment group. During heatstroke induction, we also observe the effects of pretreatment with high concentration SAH on levels of free radicals and cytokines in rats. The similar results were also obtained, the plasma levels of free radicals and cytokines were significantly expressed in advance in rats of high concentration SAH group. Meanwhile, the DNA damage of tissue was also obtained ahead of time in high concentration SAH treatment rats during heatstroke. According to our results, we speculated that animals with high levels of SAH pretreatment may probably be one of high risk factors to early induce heatstroke formation, and beforehand reveal or aggravate the heatstroke- induced damage.