Micro-RNAs Express Differentially in Glioblastoma Multiforme and Normal Brain Cells

• Main Authors: Hao-Yu Chuang, 莊皓宇
• Other Authors: 林欣榮
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/09681768921666467965

碩士 === 中國醫藥大學 === 臨床醫學研究所碩士班 === 97 === Object We hypothesize that Micro-RNAs might play an important roles in glioblastoma glastoma (GBM) cells, and differentially express between GBM and normal brain cells. We compare clinical datas using MicroRNA Chip Data Analysis and statistical analyses by use of the algorithm in miRBase. Methods We compare clinical datas using MicroRNA Chip Data Analysis and statistical analyses by use of the algorithm in miRBase. After surgical resection, we make definite GBM pathological specimen examination and to culture GBM cells. The normal brain tissue was donated from a patient expired with intracranial lesions, and we excise the normal brain tissue within 30 minutes just after his death. We want to find some differently expressed miRs (If miRNA intensity<1, adjust to 1, Ratio=log2 (case#1/case#2) ) in these 4 types of cells, and adopt to molecular- biological research for malignant brain tumors. Furthermore, we also adopt our preliminary datas to compare with Gene expression assay datas of Normal Brain Tissue ( GDS596 ) from Affymetrix U133A and GBM primary culture cell lines (Affymetrix U133 Plus 2.0) Results We have obtained a promising preliminary miRNA profiling which resulted from comparing with GBM and non-tumor brain tissues. By use of the algorithm in miRBase Targets, some miRNAs have been computationally predicted to show the significantly change of expression ratio in our preliminary miRNA data. Conclusion Some Micro-RNAs significantly expressed differentially in GBM cells and normal brain cells. Some express as up-regulation, and others express as down-regulation. Further study including Micro-RNAs RT- PCR and gene expression assay are ongoing. Micro-RNAs might play an important