Confirmation of resistance to fusidic acid among clinical isolates ofStaphylococcus aureus-preliminary report from Taiwan

• Main Authors: Mei Huang, 黃梅
• Other Authors: 王任賢
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/42688898498869665894

碩士 === 中國醫藥大學 === 臨床醫學研究所碩士班 === 97 === Abstract The number of Staphylococcus aureus with fusidic acid and methicillin resistance significantly increased from 2002 to 2007 in Tungs’ Taichung MetroHarbor Hospital, a 1405-bed district teaching hospital in central Taiwan. We conducted this study to investigate the mechanisms of resistance to fusidic acid in clinical isolates of methicillin-resistant S. aureus (MRSA). Thirty three clinical isolates of fusidic acid-resistant MRSA were collected between December 2007 and August 2008. Fusidic acid-resistant isolates were probed for the presence of the horizontally acquired resistance determinants fusB and fusC by a multiplex PCR assay. Mutations in the gene encoding the drug target (fusA) were detected by PCR and DNA sequencing. Resistant isolates were subjected to analysis by multilocus sequence typing (MLST) and SCCmec typing . The most common resistance determinant was fusC, found in 23 of the 33 isolates. One of 23 strains harboured fusB and fusC simutaneously. Ten strains without fusB or fusC have either fusA mutation in H457Y, G556S, or R659L. Molecular typing of SCCmec typing showed that all 33 isolates carried type III SCCmec elements. MLST showed this 33 isolates are ST239. Pusle field gelelectrophoresis analysis revealed a cluster with 8 different patterns. This study suggests a high prevalence of the fusC in in clinical isolates of fusidic acid-resiatant MRSA