| Summary: | 博士 === 中國醫藥大學 === 中國醫學研究所博士班 === 97 === Construction and Application of Whole
Genome Expression Profile Database for
Chinese Medicinal Herbs
Wen-Yu Cheng
Adviser: Tin-Yun Ho
Graduate Institute of Chinese Medical Science, China Medical University
PURPOPSE: The objective of this study is to construct the whole genome expression profile database for Chinese medicinal herbs and its application on the virtual screening of new drug development.
METHODS AND MATERIALS: HepG2 cells were treated with 15 formulae, SHXXT and its components, vanillin, and quinoclamine, and the gene expression profiles were analyzed by DNA microarray.
RESULTS: Firstly, our results showed that formulae displayed different expression profiles of the toxicology-related genes with chemicals, suggesting that the toxicities of 15 formula treatments in kidney might be ignored. Secondly, gene set enrichment analysis indicated that SHXXT and its components displayed a unique antiproliferation pattern via p53 signaling, activated, and DNA damage signaling pathways in HepG2 cells. Network analysis showed that most genes were regulated by p53. Thirdly, genes down-regulated by vanillin were associated with cellular process, cell cycle, death, and cancer progression. Fos may play a central role in the regulation of the gene expression network. Analysis of Fos-related transcription factor, activator protein 1 (AP-1), showed that vanillin inhibited AP-1 activity in a dose-dependent manner. Furthermore, vanillin regulated AP-1 activity via extracellular signal-regulated protein kinase pathway. Finally, quinoclamine-regulated genes interacted with nuclear factor-κB or its downstream genes by network analysis. Moreover, these genes were involved in cell cycle and apoptosis by gene ontology annotation.
CONCLUSION: The results of this study showed a perspective of constructing the whole genome gene expression profiles database for Chinese medicinal herbs and its application on the virtual screening of new drug development.
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