The expression of hepcidin and erythropoietin receptor are affected by Vitamin C and Erythropoietin in HepG2 cell

• Main Authors: Ping-Fanf Chiu, 邱炳芳
• Other Authors: 柯順耀
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/14290856775232907902

碩士 === 長榮大學 === 醫學研究所 === 97 === The hepcidin, newly found protein secreted by hepatic cell, modulate the de novo iron absorbed from duodenum and the recycled iron released from reticuloendothelial system. Uremia patients with higher serum hepcidin level is proved as in more severe anemic state. The administration of higher erythropoietin (EPO) dose or Vitamin C enable to correct those functional iron deficiency. But EPO is very expensive and limited dosage . The EPO regulated hepcidin expression within HepG2 cell and murine hepatocyte is identified by Pinto et al. recently. However, the mechanism of Vitamin C to improve anemia is still unknown. That study is supposed that Vitamin C may regulate hepcidin expression as EPO. HepG2 cell was cultured with Vitamin C (0-100 ?慊/ml ) and EPO (0-1.0 U/ml) separately for 6 hours at 37℃. Reverse transcription polymerase chain reaction and real time PCR were to measure hepcidn , EPO and EPO-R expressions. The result reveals that EPO can inhibit the hepcidin expression within HepG2 cell in dose-dependency. Besides, Erythropoietin will down-regulate its receptor in HepG2 cell early. Moreover, It is excitedly that Vitamin C is found to enhance the EPO-R expression. Vitamin C has little effect on EPO expression. That may be account for and offer one mechanism of Vitamin C to improve the anemia is mediated by its enhanced expression of EPO-R, that reduces the production of hepcidin eventually. Those result will offer power of Vitamin C for anemia care.