Over-expression of ADP-ribosylation factor 1 in Human Gastric Carcinoma and its Clinicopathological Significance

• Main Authors: Yi Feng Cheng, 鄭義奮
• Other Authors: K. H. Lin
• Format: Others
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/63717760147750770025

碩士 === 長庚大學 === 生物醫學研究所 === 97 === Gastric cancer is the fifth leading cause of cancer-related death in Taiwan. Identification of gastric cancer related factor is essential to increase patient survival. ADP ribosylation facor 1 (Arf1) was identified using two-dimensional electrophoresis (2-DE) combined with Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometer (MALDI-TOF) and isobaric tag for relative and absolute quantitation (iTRAQ). ARF1 belongs to ARF family and Ras superfamily. It is a small guanine nucleotide-binding proteins that enhance the enzymatic activities of cholera toxin. The small ADP ribosylation factor (ARF) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular traffic and functioning as activators of phospholipase D. The expression of ARF1 mRNA was up-regulated in tumor tissues (compared with adjacent non-tumor tissues) of about 67.2% gastric patients. The expression of ARF1 protein was also identified by western blot and Immunohistochemistry (IHC). The clinic-pathological correlation of ARF1 was valuated. Elevated ARF1 expression was strongly correlated with depth of invasion, lymph node metastasis, and pathological stage. In order to understand the relationship between ARF1 over-expression and gastric cancer, ARF1 over-expression stable clones were established. Experimental results indicate that over-expression stable clones can enhance cell proliferation, migration, invasion. These results indicate that ARF1 over-expression may contribute the poor prognosis of patients. However, the underlying mechanism are waiting for further study.