| Summary: | 碩士 === 長庚大學 === 生物醫學研究所 === 97 === Honeybees (Apis mellifera) have served as an animal model for the study of aging. The lifespan of queen bees is 12-fold longer than that of worker bees.This study differentiates the differences of age-related molecules between worker bees and queen bees by using histochemical, biochemical, and genetic techniques.
The results showed that senescence-associated -galactosidase, lipid peroxidation, protein oxidation, glutathione peroxidase activity and catalase activity were increased with age, whereas SirT1 expression is decreased with age in worker bees and queen bees. However, telomerase activity and telomere length have no significant difference.
The results also showed that the expression of some age-related molecules was different between worker bees and queen bees. Reactive oxygen species(O2‧- and H2O2), superoxide dismutase activity(MnSOD and Cu/ZnSOD) ,Catalase expression are decreased with age, whereas the expression of FoxO, MnSOD and JNK was increased with age in worker bees. Besides the expression of Lipofuscin granules, JNK and Catalase expression has no significant difference with worker bees, the expression of remainder is reversely proportioned to worker bees in queen bees.
Collectively, some age-related molecules expressed similar tendencies; others did different tendencies between worker bees and queen bees. The different expression (Waste accumulation,ROS level,Antioxidant enzyme efficiency,Gene regulation)of age-related molecules between worker bees and queen bees laid a foundation to further study the mechanism of aging and anti-aging.
|
|---|
