Translational regulation of pri/tal by Drosophila EGFR pathway

• Main Authors: I chung Chen, 陳怡君
• Other Authors: H. W. Pi
• Format: Others
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/25563685997291873617

碩士 === 長庚大學 === 生物醫學研究所 === 97 === pri/tal was identified as a polycistronic gene in Drosophila and it has important roles in epithelial morphogenesis and pattern formation in Drosophila. Based on our in situ hybridization data, we suggest that pri/tal may be regulated by the EGFR signaling. Thus, I want to futher study between pri/tal and the EGFR regulative relations for the positive regulation or negative regulation. We knew that EGFR pathway is essential for many development processes in Drosophila, including chordotonal organ recruitment, wing vein formation, and follicle cells patterning, etc. Therefore, we use the genetics and in situ hybridization method proved that EGFR signaling regulated pri/tal in follicle cells and chordotonal organ. But, we also found that when overexpressed EGFR transcription repressor Yan, pri/tal was ectopically expressed in eye disc. Thus, we suggested that pri/tal expression is regulated by EGFR signaling in different tissue. EGFR signaling has two transcription factors, Pointed and Yan and they belong to Ets family and they can recognize the ETS binding site. Therefore I use the ETS binding site to search for pri/tal regulatory region, and analyzes these regulatory region whether to regulate by the EGFR signaling. Here we reported that pri/tal upstream 5k region including leg disc pretarsal region, presumptive joint, antennal segment, follicle cells and embryo tracheal precursor, and so on different expression. We use the genetics method to porve that the 2.9kb pri/tal regulatory region in follicle cells, indeed regulated by EGFR signaling. Therefore, pri/tal 2.9k reporter was regulated by the EGFR signaling in the follicle cells.