Chemical and Biological Investigations of Bupleurum kaoi Liu

• Main Authors: Jia-Wei Chen, 陳嘉緯
• Other Authors: Y. L. Leu
• Format: Others
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/00145218405328035592

碩士 === 長庚大學 === 天然藥物研究所 === 97 === Bupleurum kaoi is the only species of Bupleurum reported from Taiwan, it is also perennial aquatic herbaceous plant and mainly distributed in hill of north and midland Taiwan. Forty-three compounds were isolated from the dry B. kaoi. Thirty-three of these compounds were determined by spectral analysis and compared with authentic literatures. They included benzoic acid, pyrocatechol, pyrogallol, 4-hydroxybenzoic acid, methyl vanillate, maltohexaose, paraxanthine, caffeine, L-tryptophan, L-tyrosine, levodopa butyl ester, adenosine, guanosine, genistein, isorhamnetin, rutin, isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-glucoside, (-)-epicatechin-3-O-gallate, (-)epigallocatechin-3-O-gallate, BKC 17-6C, chinesin, 5-(4-hydroxy-3-methoxyphenyl)furo[3’,4’:6,7]naphtho[2,3-d]-1,3-dioxol- 6(8H)-one, isodiphyllin, 9-(3,4-dimeth-oxyphenyl)-5,9-dihydro-8H-furo[3',4':6,7]naphtha[2,3-d][1,3]dioxol-6-one, saikosaponin a, saikosaponin b1, saikosaponin b2, 6"-O-acetylsaikosaponin b2, 23-O-acetylsaikosaponin b2, saikosaponin b3, saikosaponin g, 1,3-dihydroxypropan-2-yl palmitate. The isolated compounds were evaluated for anti-hepatitis B surface antigen (HBsAg) expression and cytotoxicity. HBsAg-transfected human hepatoma cells (Hep3B cells) cultured with saikosaponin b2 at 100 μg/ml showed significantly lower level of HBsAg in culture medium and no cytotoxicty to normal cell.