| Summary: | 碩士 === 國立中正大學 === 機械工程所 === 97 === The ability to produce patterns of single or multiple cells is essential in the bioassays. The standard photolithography is employed in the present study to manufacture the single-cell-based microarray chip. The surface of the microchip was modified by silane and patterned by the polymer material, PEGDA. The cell colonies of three cytological grade bladder carcinoma cell lines (TSGH-8301、J82、TCC-SUP) were used to study the thermal injury in the first place. The parameters of activation energy and frequency factor in Arrhenius model, which is the well-known model to describe the thermal injury of cells, for the bladder cancer cell lines were also evaluated for a quantitative confirmation. Accordingly, the experimental data showed that TCC-SUP cells and TSGH-8301 cells exhibited the highest and lowest thermal sensitivities, respectively. Moreover, the HeLa cells were then patterned on the microchip to investigate the mechanism of thermotherapy (hyperthermia). The single-cell-base microchip is suitable to be used in the biomedical applications with single-cell resolution.
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