Deformability of Micron-Size Soft-Matter Measured by Dual

• Main Authors: Jar-Jin Bai, 白佳巾
• Other Authors: Arthur Chiou
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/12574350094150491778

碩士 === 國立陽明大學 === 生醫光電工程研究所 === 96 === In cell mechanics, or equivalently, the cellular level of biomechanics, an understanding of how a living cell responds to external physical, chemical, and other environmental stimulants, including morphological deformation under external forces, may shed light in the research and development of new techniques for biomedical diagnosis, therapy and drug efficacy assessment. This thesis focuses on the applications of a pair of parallel twin tweezers to trap and stretch a micron-size soft particle by increasing the distance between the focal spots (or the trap centers) of the two traps. Specifically, we measured and compared the fractional deformation of a wide range of compliant particles as a function of the distance between the two focal spots of the twin tweezers. The sample particles studied in this project include human red blood cells (RBCs), osmotically swollen into a micro-sphere, with and without treating with N-ethylmaleimide (NEM), and at different temperatures, as well as liposomes with different DOPC/cholesterol ratio (1:1 vs.1:3) For spherical RBCs, smaller deformation were observed in samples at room temperature with NEM treatment and samples at 42 ℃without NEM treatment, compared with the deformation of the control set, namely, RBCs at room temperature without NEM treatment. The speculation that high temperature (42 ℃) and NEM treatment could lead to similar changes to cytoskeleton, such as the transformation of spectrin from tetramer to dimmer requires further study