Imaging Dental Section with Polarization- resolved SHG and Time-resolved Autofluorescence

• Main Authors: Chung-Haung Chen, 陳俊熿
• Other Authors: Fu-Jen Kao
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/00368906548842257820

碩士 === 國立陽明大學 === 生醫光電工程研究所 === 96 === In this study, we have developed a high performance multi-photon microscopic system and combined with FLIM (fluorescence lifetime imaging microscopy). We can not only get more space-solved information about samples but also explore more time-solved information about samples. In this system, we ensure imaging reality and accuracy. By nonlinear optical theorem, we perform second-harmonic generation, auto-fluorescence, and auto-fluorescence lifetime imaging on an nonlinear-material of PPLN, a collagen, and a tooth. We use this system in order to explore the correlation between polarization with second harmonic generation, auto-fluorescence, and auto-fluorescence lifetime imaging.