Proteome analysis of Streptomyces avermitilis upon mitomycin C treatment

• Main Authors: Bo-Sin Zeng, 曾博新
• Other Authors: Chao-Hsiung Lin
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/56729445217367574641

碩士 === 國立陽明大學 === 生命科學暨基因體科學研究所 === 96 === Streptomyces is the largest genus of actinobacteria. The members of streptomyces genus are more than 500. Streptomyces like other actinobacteria carries bigger genome and higher G+C content. Streptomyces boarded in soil with slowly growth. Streptomyces is remarkable microorganism for containing complex and plenty of secondary metabolite. This advantage can make Streptomyces become dominated organisms. The "earthy" odor is come form Streptomyces production of a volatile metabolite, geosmin. Streptomyces is very popular material for bacterial genetic, because of their chromosome is linear and instability. Streptomyces avermitilis is an industrial microorganism. Avermectin, anthelmintic macrolide is the major product from S. avermitilis. Avermectin can bind insect GABA receptor with result that Cl- channel opened. Higher concentration Cl- flowed into neuron which cases hyperpolarization of the nerve or muscle cell. The result is disconnecting neuron from muscles which process the insect death. Mitomycin C is an effective DNA crosslinker. The crosslinking accomplish by by two N-alkylations. Both alkylations are sequence specific for a guanine nucleoside in the sequence 5'-CpG-3'. The result is DNA replication stopping which promote bacterial death. Because of industrial value the genome has been completely sequenced in 2003. In this study, we used two-dimensional gel electrophoresis to separate solute protein from S. avermitilis under mitomycin C treatment. To date, 63 proteins have been identified. Those proteins participate in many pathways such as DNA repair, Purine and pyrimidine metabolism, pentose phosphate pathway, glutathione biosynthesis and antioxidant system. Details of S. avermitilis biosynthesis and regulatory mechanism remain unclear. This study may be a first proteomic research of S. avermitilis with protein identifications.