| Summary: | 碩士 === 國立陽明大學 === 生命科學暨基因體科學研究所 === 96 === Because of bamboos’ long junvenile period, culture of germ-free bamboos is one important way to solve damages by viral infection. During tissue culture, some plantlets become albino and can not grow in the field. Therefore, we hope to understand the reason of albinim. There are several possible reasons to cause the albino mutant formation, including large deletion of chloroplast genome、abnormal gene expression and defects in post-transcription modification. In this study, genome integrity, gene expression of plastid genes and RNA splicing of three albino mutans of Bambusa edulis which are named as ab, ab1 and ab2, are characterized by sequencing, Southern blot, RT-PCR and northern blot.
Firstly, the Bambusa oldhamii chloroplast genome has been sequenced by the PCR method. Comparing the genome of Bambusa oldhamii with that of Oryza sativa; location of ycf15 gene and the sizes of IR (inverted repeats) are different. But the gene content and other gene order are highly conserved between the bambusa and oryza chloroplast genomes. 61 chloroplast highly conserved genes from the 38 species are used to construct the Maximum Parsimony (MP) phylogenetic tree. The MP phylogenetic tree shows that the Bambusa and Oryza are very closely related.
Information of sequence has bee applied to design primers for detecting the genome integrity in three Bambusa edulis albino mutants, the genomic PCR and Southern blot were used. There are large deletions of plastid-encoded genes in ab albino mutant. Even ab2 and ab1 albino mutants show few deletions in their plastomes. Gene expression of many plastid-encoded genes was different from that in normal plants. Comparing the sizes of genes in the genome with their gene products, rpl2、atpF and ndhA are not correctly spliced in the albino plants. In summary, albino mutants have gene deletions, aberrant gene expression and the defect of intron splicing have been observed in.
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