| Summary: | 碩士 === 國立陽明大學 === 公共衛生研究所 === 96 === Human Cytomegalovirus (HCMV), a ubiquitous virus around the world, belongs to the Betaherpesvirinae subfamily of Herpesviridae. Virus signal can be detected in most adults, and it usually establishes an asymptomatic or sub-clinical infection. However it can cause severe complications in congenitally infected infants and immuno- compromised patients with virus reactivation. Previous studies showed that specific HCMV genotypes were related to tissue-tropism and disease. The glycoprotein B (gB) and glycoprotein N (gN) were known to induce neutralizing antibodies. Their genes were hypervariability but were highly conserved within herpesviridae. The object of this study is to understand the distribution of HCMV genotypes in the population of Taiwan. We therefore analyze the UL55 (gB) and UL73 (gN) genotype variability of clinical HCMV in Taiwan. We collected HCMV clinical isolates from Taipei Veterans General Hospital for genotyping. Genotype of HCMV was carried out by Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) analysis combined with partial sequencing. Phylogenetic analysis was performed to analyze the genetic variation. For a total of 108 isolates during 2005 to 2006 year, the genotype distribution of the gN were: 28 (25.9%) type 1, 13(12.0%) type 2, 16(14.8%) type 3a, 7(6.5%) type 3b, 7(6.5%) type 4a, 9(8.3%) type 4b, 18(16.7%) type 4c, 9(8.3%) mixture,1(0.9%) non detected; as for the gB only types 1 and type 3 but not types 2 and type 4 were detected, and there were 76(70.37%) type 1, 28(26.0%) type 3, 3(2.8%) mixture of type 1 and 3, 1(0.9%) non detected. The objective of this study is to understand the genetic variability of HCMV in Taiwan. When compared the local genotypes of genetic variants of gB and gN which those around the world, our results showed that gN genotypes randomly exist in Taiwan’s population but gB genotype only exist in gB1 and gB3. We may further study whether the genotype distribution is influenced by geography and race.
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