Research of DNase encapsulated by PLGA/Liposomes microspheres

• Main Authors: Yao-Hui Tsai, 蔡耀輝
• Other Authors: Chung-Yih Wang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/90603576512368038063

碩士 === 大同大學 === 生物工程學系(所) === 96 === The traditional drug delivery systems include pills, capsules, or injection. In this study, A novel drug delivery system utilizing poly (DL-lactide-co- glycolide)(PLGA) encapsulation technology was developed. Deoxyribonuclease (DNase) was encapsulated in egg yolk phosphatidylcholine (EYPC) liposomes with stabilizers, such as charged lipids, astaxanthin and trehalose. The result showed that DNase stabilized in CaCl2 solution and then encapsulated with EYPC liposomes showed the highest encapsulation efficiency(45.13%) and enzyme activity(43.79%). DNase-liposomes was then encapsulated in PLGA microspheres. PLGA microspheres containing DNase, which stabilized with CaCl2, and encapsulated with astaxanthin, and trehalose stabilized liposomes, showed the highest DNase activity after release. The accumulated DNase activity can reach 32.56 unit/mg microspheres. The microspheres were then cultured with macrophages. Macrophages phagocytosis was not significant in the primary stage. After 12 hr culture, about 22.84 % of microspheres were phagocyted by macrophages. We found that macrophages phagocytosis stopped when 43% microspheres were engulfed. The viability of macrophages was 90.41%, after 48 hr culture, suggesting the microspheres will not lead to death of macrophages.