THE STUDY OF MOLECULAR BIOLOGY METHOD FOR THE GASTROENTERITIS SYNDROME IN STOOL

• Main Authors: Yu-Fen Tai, 戴鈺芬
• Other Authors: Chien-Hsien Chen
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/70895399894025090663

碩士 === 大同大學 === 生物工程學系(所) === 96 === ABSTART Adenovirus, rotavirus, norovirus, astrovirus, and enterovirus are the major viruses in causing acute gastroenteritis among human beings. Especially for children is higher risk to infect than adults. Therefore, to evaluate the major parameter to effect on acute gastroenteritis, we collect stool samples from northern to southern Taiwan of 270 fecal specimens of diarrhea patients, since July 2005 until July 2007. The study collection specimen’s direction method used ELISA, RT-PCR, Seminest-PCR. Among 270 fecal specimens collected from diarrhea patients, 50 (18.5%) were positive for rotavirus, 48(17.7%) were positive for norovirus and 9 (3.3%) patients were detected as positive for adenovirus. Besides, dual infections were found in 5.9 % (16/270) of the positive samples for two of three enteric viruses. There were five diarrhea cases with rotavirus/norovirus GI infection, and four cases with rotavirus/norovirus GII. Almost above of 60% gastroenteritis virus positive were collected from children under age 10, there were 60%, 88.8%, 81.8% and 67.7% positive for rotavirus, adenovirus, norovirus GI, norovirus GII, respectively. According to the ANOVA test method, it showed that there were strong statistic relationship between virus infection and patient age. However there was no significant difference for virus infectious frequency with geographical region. The seasonal trends of norovirus detection were characterized by a peak incidence during spring (31.8 %), whereas adenovirus and rotavirus diarrhea occurred with summer and autumn, respectively. An epidemic of acute gastroenteritis was also identified as peaking mainly in July-September (>15 cases per month). The purpose of this study is to develop one reliable detection method by applying molecular biology method for the gastroenteritis. In this study, we combined RT-nested-PCR and molecular epidemiological method to detect and identify total 270 suspected gastroenteritis cases. The result showed that Seminest -PCR method were more efficiently to detect these three enteric virus. According to the result of analysis of acute gastroenteritis cases, there were strong significant relationship between gastroenteritis cases and virus infectious frequency (ANOVA test, p<0.05). Total 107 clinical samples of gastroenteritis virus from 2005 July to 2007 July were analyzed by sequence analysis and then identify serotype. The result showed that there were rotavirus G1, G3, G9 serotype ; Adenovirus type 2, 3, 41 ; Norovirus GI/8 and GII/4 detected in this study. In this study, it appeared that Taiwan epidemic gastroenteritis virus strains were closely related with other East Asian epidemic virus strains. It suggested that there were many epidemic gastroenteritis virus strains prevalent in East Asian region include Taiwan since 2005. Key Words: Rotavirus, Adenovirus, Novovirus