| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 96 === Haloferax mediterranei is used in this study as an extremely
halophilic archaebacterium for red pigment (hmERP) production. In
this study, we investigate the composition, characteristic structure and
molecular weight of components in hmERP. The optical properties of
hmERP were analyzed by UV/VIS Spectrophotometer, and shown that
the absorption wavelength of hmERP are 527, 494, 468, 388 and 371
nm with maximum wavelength at 494 nm. We also found that the
absorption wavelength of hmERP decayed regularly under light
irradiation. From the infrared absorption spectrum, the molecular
structure of hmERP contain hydroxy (-OH) and C=C double bond.
After separating by reversed-phase High Performance Liquid
Chromatography (HPLC) at C18 column with 70% acetone and 30%
water as the mobile phase, the hmERP contains at least six
compounds. The molecular weight 741.6(M+H)+ of peak A (major
peak with shortest retention time) in hmERP was obtained by
LC-APCI-MS. Based on the IR spectrum, molecular structure and
molecular weigh analysis, we conclude that component in peak A of
hmERP is same as Bacterioruberin (a C50 carotenoid derivative).
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