| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 96 === Caco-2 cells cultured on polycarbonate membrane can be spontaneously differentiated to close cell monolayer which is the same as gastrointestinal epithelial cells. Caco-2 cells as a model system can conquer the defects of using a lot of drugs for absorption and metabolism of animals. Hence, they can be used for sifting application of medicine which is absorbed in gastrointestinal tracts. Liposomes have some properties, such as good biocompatibility biodegradation, anti-immunization, and can be used for encapsulation of fat-soluble and water-soluble drug. Hence, they can be good carriers for drug delivery. In this study, liposomes for lavastatin was made by DMPC (Dimyristoyl-sn-Glycero-3-Phosphocholine) or EYPC (Egg-yolk L-α-Phosphatidylcholine) and permeation test of lovastatin by Caco-2 as a model system was studied. The vesicle volume distribution, entrapment efficiency, and cytotoxicity were analyzed and drug permeation studies through the Caco-2 cell were studied. Lipsomes were co-cultured with Caco-2 cells and significant cytotoxicity was not found. It is found that the entrapment efficiency of DMPC liposomes is 95.2 ± 0.54 (％), and EYPC liposomes is 86.76 ± 4.71 (％) when the lipids and the drugs got a ratio of 1:0.25 (mol) via HPLC Analyzer. The drug permeation of DMPC and EYPC liposomes through a monolayer made by Caco-2 cells are 5.62 and 5.9 times better than the permeation of pure drug . The result was tested using the lipsomes made by lovastatin and pure drug of lovastatin going through a monolayer made by Caco-2 cells.
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