Application of self-microemulsification solvent diffusionmethod for preparation of PLGA nanoparticles

• Main Authors: Yu-Ling Lo, 羅又伶
• Other Authors: 許明照
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/62480310938074061250

碩士 === 臺北醫學大學 === 生醫材料暨工程研究所 === 96 === The objectives of this study were to establish a new preparation method for poly (DL-lactide-co-glycolide) (PLGA) nanoparticles by application the Self-microemulsification solvent diffusion method and to elucidate the mechanism of nanoparticle formation on the basis of the microemulsion principle of PLGA and surfactants、cosurfactants in the preparation system. PLGA nanoparticles were prepared by the utilization of dilutable Self- microemulsifying systems to form microemulsions as the template for preparing nanoparticles of PLGA.Microemulsion system wes devely with the use of partially water miscible and pharmaceutically acceptable solvents as the oil phase and the pseudo-ternary phase diagrams was constructed to characterize and optimize surfactants, cosurfactants, and cosolvents for the dichloromethane in the production of nanoparticles. Tween series (20-80), were selected as surfactants for preparations of nanoparticles. cosurfactants were selected from short-chain alcohols (ethanol, propanol, and butanol) for preparations of nanoparticles. The pseudo-ternary phase diagram were constructed for optimal combinations of selected solvents for nanoparticles were surfactants/cosurfactants and water/cosolvents. These systems will be examined and process parameters was optimized.